Fig 1: TRIM5γ Inhibits HBV Infection and Replication by Targeting HBx(A) HepG2 cells were co-transfected with constructs expressing TRIM5γ proteins and either pHBV1.2 WT or DX, as indicated. After 72 h, cells and supernatant were harvested and subjected to qPCR or ELISA. (B) Scheme of HBV infection. (C) HepG2-NTCP cells were infected with lentiviral-ctrl or lentiviral-TRIM5γ for 16 h, and later, cells were washed with PBS and inoculated for 24 h with HBV. After infection, cells were washed three times with PBS and were maintained for another 9 days; the medium was changed every 2 days. The supernatant and cells were collected for detection of HBV DNA, pgRNA, and HBsAg by qPCR or ELISA. (D) HepG2-NTCP WT or TRIM5α/γ KO cells were infected with HBV for 24 h with or without IFN-α treatment (10 ng/mL) and analyzed as in (C). Mean ± SD values from three independent experiments are shown. *p < 0.05, **p < 0.01, ***p < 0.001.